# First Commission Directive of 13 November 1979 laying down Community methods of analysis for testing certain partly or wholly dehydrated preserved milk for human consumption (79/1067/EEC), Division 3: PRINCIPLE

Extent: E+W+S+N.I.

The reconstituted sample is diluted with a buffer substrate at pH 10,2 and incubated at a temperature of 37 oC for two hours. Any alkaline phosphatase present in the sample will, under these circumstances, liberate p-nitrophenol from added disodium p-nitrophenyl phosphate. The p-nitrophenol liberated is determined by direct comparison with standard colour glasses in a simple comparator using reflected light.

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Source: https://www.legislation.gov.uk/eudr/1979/1067/annex/II/division/9/division/3 (the official version)
Page: https://openlegislation.uk/eu-directive-1979-1067/annex-II/division-9/division-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.