Division 6.1 Standardization of the Fehling's solution

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1. 6.1.1Division 6.1.1
2. 6.1.2Division 6.1.2
3. 6.1.3Division 6.1.3
4. 6.1.4Place the boiling flask on the heating device (5.5), previously adjusted so that boiling commences in 120 ± 15 seconds.
5. 6.1.5Division 6.1.5
6. 6.1.6Boil the contents of the flask for 120 seconds, as timed by the stop-watch.
7. 6.1.7After boiling has continued for 120 seconds (by the stop-watch) start adding standard dextrose solution to the boiling flask (5.1) from the burette (6.1.2) in 0·5 ml increments until the colour of the methylene blue is discharged (see notes 2 and 3).
8. 6.1.8Division 6.1.8
9. 6.1.9Division 6.1.9
10. 6.1.10Division 6.1.10
11. 6.1.11After boiling has continued for 120 seconds (by the stop-watch), start adding standard dextrose solution to the boiling flask (5.1) from the burette, initially in 0·2 ml increments and finally dropwise, until the colour of the methylene blue is just discharged.
12. 6.1.12Division 6.1.12
13. 6.1.13Vo shall be between 19·0 and 21·0 ml standard dextrose solution (4.3).
14. 6.1.14Division 6.1.14
15. Note 1:
16. Note 2:
17. Note 3:
18. Note 4: