Division 5.3.2 Preparation of the standard

Into a 50 ml volumetric flask, accurately weigh about 500 mg of nitromethane (5.1.4) and either 500 mg of chloroform (5.1.1) or 210 mg of 2,4-dimethylheptane (5.1.2). Make up to volume with 95 % ethanol (5.1.3). Mix well. Place 5 ml of this solution into a 20 mg volumetric flask. Make up to volume with 95 % ethanol (5.1.3).

Inject about 10 μl using the specified microsyringe (5.2.2). Make five injections.