Division 5.3.3.1 Column

This is in two parts, the first containing didecyl phthalate on Gas Chrom Q as packing, the second having Ucon 50 HB 280X on Gas Chrom Q as packing. The prepared combined column must yield a resolution ‘R’ equal to, or better than, 1,5, where:

$$\mathrm{R=\; 2}\frac{\mathrm{d\text{'}}\left(r_2{\mathrm{-\; r}}_1\right)}{W_1{\mathrm{+\; W}}_2}$$

let:

r₁ and r₂

retention times (in minutes),

W₁ and W₂

peak widths at half height (in millimetres),

d'

the chart speed (in millimetres per minute).

As examples the following two parts yield the required resolution:

Column A:

Material: stainless steel.

Length: 1,5 m.

Diameter: 3 mm.

Packing: 20 % didecyl phthalate on Gas Chrom Q (100 to 120 mesh).

Column B:

Materia: stainless steel.

Length: 1,5 m.

Diameter: 3 mm.

Packing: 20 % Ucon 50 HB 280X on Gas Chrom Q (100 to 120 mesh).