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Division 5.2.7.8 The conditions of chromatography are given as a guide. They permit the achievement of a resolution ‘R’ equal to, or better than, 1,5, where:

United Kingdom

Original text. Later amendments are not shown.

R = 2 d' r 2 - r 1 W 1 + W 2

let:

r1 and r2

  • retention times (in minutes),

    • W1 and W2

  • peak widths at half height (in millimetres),

    • d'

  • the chart speed (in millimetres per minute).

    • It is recommended that chromatography be terminated by regulating the tempera-ture from 90 to 150 oC at a rate of 10 oC per minute so as to eliminate substances liable to interfere with subsequent measurements.