Division 9.1 Calculating a response factor of a substance ‘p’ relative to a substance ‘a’ selected as an internal standard

Let the first substance be ‘p’, where:

k_p

its response factor,

m_p

its mass in the mixture,

A_p

its peak area.

Let the second substance be ‘a’, where:

k_a

its response factor (made equal to unity),

M_a

its mass in the mixture,

A_a

its peak area,

then:

$$k_p=\frac{m_p{\mathrm{\times \; A}}_a}{M_a{\mathrm{\times \; A}}_p}$$

As examples the following response factors have been obtained (for chloroform: k = l):

Dichloromethane: k₁ = 0,78 ± 0,03

1,1,1-trichloroethane: k₂ = 1,00 ± 0,03