Division 6.1 Hydrolysis

Weigh accurate y into a 50 ml round-bottom flask approximately 1 g of the sample (m). Add 5 ml of water and 5 ml of hydrochloric acid (4.3) and boil for one hour, using a reflux condenser. Immediately transfer the hot suspension with water into a 50 ml graduated flask. Allow to cool and make up to the mark with water. Centrifuge at at least 3000 rpm for five minutes and pass the supernatant liquid through a filter.