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Division 4.10 Eluant: phosphate buffer solution (4.9)/methanol (4.1) 61/39(v/v)

United Kingdom

Original text. Later amendments are not shown.

The composition of the mobile phase may be changed in order to achieve a resolution factor R ≥ 1,5.

R = 2 d'R 2 — d'R 1 W 1 + W 2

where

R1 and R2

  • retention times, in minutes, of the peaks,

    • W1 and W2

  • peak widths at half height, in milimetres,

    • d'

  • the chart speed, in millimetres per minute.