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Division 5.3 Development

United Kingdom

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  1. 5.3.1Division 5.3.1
  2. 5.3.21Division 5.3.21
  3. 5.3.3Division 5.3.3
  4. 5.3.4Observe under the light from a UV lamp adjusted to a wavelength of 360 nm. Quinine appears as a fluorescent intense blue spot.
  5. 5.3.5For further confirmation that quinine is present, the plate is exposed for approximately one hour to bromine vapour (3.7). The fluorescence disappears. When the same plate is exposed to ammonia vapour (3.8), the spots reappear with a brown colour, and when the plate is again examined under UV light at 360 nm a yellowish fluorescence can be observed.