OpenLegislation

Division 4.10 Eluant: orthophosphoric acid (4.7) /potassium dihydrogenorthophosphate (4.8) /tetramethylammonium bromide (4.9)/water/acetonitrile (4.1) 10/50/100/340/90 (v/v/v/v/v).

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Original text. Later amendments are not shown.

The composition of this mobile phase may be changed in order to achieve a resolution factor R ≥ 1,5.

R = 2 d'R 2 — d'R 1 W 1 + W 2

where

R1 and R2
  • retention times, in minutes, of the peaks,

  • W1 and W2
  • peak widths at half height, in millimetres,

  • d'
  • the chart speed, in millimetres per minute.