# Council Directive of 26 June 1990 on animal health conditions governing the movement and import from third countries of equidae (90/426/EEC) (repealed), Division 2.1.2.1: Serum samples to be tested, and positive and negative control sera, are diluted 1 in 25 in PBS + 5 % (w/v) skimmed milk + 0,05 % (v/v) Tween 20, 100 μl per well. Incubate for 1 hour at 37 °C.

Extent: E+W+S+N.I.
Status: repealed

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Source: https://www.legislation.gov.uk/eudr/1990/426/annex/D/division/2/division/2.1/division/2.1.2/division/2.1.2.1 (the official version)
Page: https://openlegislation.uk/eu-directive-1990-426/annex-D/division-2/division-2.1/division-2.1.2/division-2.1.2.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.