Division 1 Compulsory routine testing must be carried out as follows:F1

all animals kept at a semen collection centre must be subjected to the following tests with negative results:

1. (a)

   as regards brucellosis, a buffered Brucella antigen test (rose Bengal test), or a cELISA or an iELISA;

2. (b)

   as regards Aujeszky’s disease:

   1. (i)

      in the case of non-vaccinated animals, an ELISA detecting antibodies to the whole Aujeszky’s disease virus or to its glycoprotein B (ADV-gB) or glycoprotein D (ADV-gD) or a serum neutralisation test;

   2. (ii)

      in the case of animals vaccinated with a gE deleted vaccine, an ELISA detecting antibodies to Aujeszky’s disease virus glycoprotein E (ADV-gE);

3. (c)

   as regards classical swine fever, an antibody ELISA or a serum neutralisation test;

the tests set out in point 1.1 must be carried out on samples taken:

1. (a)

   from all animals immediately prior to leaving the semen collection centre, or upon arrival at the slaughterhouse, and in no case later than l2 months after the date of admission to the semen collection centre; or

2. (b)

   from at least 25 % of the animals in the semen collection centre every 3 months and the centre veterinarian must ensure that the sampled animals are representative of the total population of that centre, in particular with respect to age groups and housing;

where the testing is carried out in accordance with 1.2(b), the centre veterinarian must ensure that all animals are tested in accordance with point 1.1 at least once during their stay at the semen collection centre and at least every 12 months from the date of admission, if their stay exceeds 12 months.