{
  "url": "https://openlegislation.uk/eu-directive-1991-68/annex-C",
  "json_url": "https://openlegislation.uk/eu-directive-1991-68/annex-C.json",
  "legislation": "eudr/1991/68",
  "legislation_title": "Council Directive of 28 January 1991 on animal health conditions governing intra-Community trade in ovine and caprine animals (91/68/EEC)",
  "legislation_url": "https://openlegislation.uk/eu-directive-1991-68",
  "path": "annex/C",
  "kind": "annex",
  "number": "C",
  "heading": "Brucellosis (B. melitensis) tests",
  "text": "For a holding to qualify for brucellosis-free status, testing for brucellosis (B. melitensis) is performed by means of the Rose Bengal method or by the complement-fixation method described in the Annex to Decision 90/242/EEC or by any other method recognized in accordance with the procedure laid down in Article 15 of this Directive. The complement-fixation method is used for tests on individual animals.\nWhen carrying out the Rose Bengal test, if more than 5 % of the animals on a holding show a positive reaction, a further test is carried out on every animal on the holding by means of the complement-fixation method.\nSerum containing 20 or more ICFT units/ml must be regarded as positive in the complement-fixation test.\nThe antigens used must be approved by the national laboratory and must be standardized against the second international standard anti-brucella abortus serum.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1991/68/annex/C",
    "retrieved_at": "2026-11-01T15:23:26.306210+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}