# Council Directive of 28 January 1991 on animal health conditions governing intra-Community trade in ovine and caprine animals (91/68/EEC), Annex C: Brucellosis (B. melitensis) tests

Extent: E+W+S+N.I.

For a holding to qualify for brucellosis-free status, testing for brucellosis (B. melitensis) is performed by means of the Rose Bengal method or by the complement-fixation method described in the Annex to Decision 90/242/EEC or by any other method recognized in accordance with the procedure laid down in Article 15 of this Directive. The complement-fixation method is used for tests on individual animals.
When carrying out the Rose Bengal test, if more than 5 % of the animals on a holding show a positive reaction, a further test is carried out on every animal on the holding by means of the complement-fixation method.
Serum containing 20 or more ICFT units/ml must be regarded as positive in the complement-fixation test.
The antigens used must be approved by the national laboratory and must be standardized against the second international standard anti-brucella abortus serum.

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Source: https://www.legislation.gov.uk/eudr/1991/68/annex/C (the official version)
Page: https://openlegislation.uk/eu-directive-1991-68/annex-C
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.