Annex D Official contagious epidydimitis (Brucella ovis) test

Complement-fixing test:

The specific antigen used must be approved by the national laboratory and must be standardized against the international standard anti-brucella ovis serum.

The working serum must be standardized with the international standard anti-brucella ovis serum prepared by the Central Veterinary Laboratory, Weybridge, Surrey, United Kingdom.

A serum containing 50 or more International Units per/ml must be regarded as positive.