# Council Directive 92/65/EEC of 13 July 1992 laying down animal health requirements governing trade in and imports into the Community of animals, semen, ova and embryos not subject to animal health requirements laid down in specific Community rules referred to in Annex A (I) to Directive 90/425/EEC, Division 1: Collection and processing of in vivo derived embryos

Extent: E+W+S+N.I.

In vivo derived embryos shall be conceived as a result of artificial insemination with semen meeting the requirements of this Directive and shall be collected, processed and preserved in accordance with the following:
1.1. Embryos shall be collected and processed by an approved embryo collection team, without coming into contact with any other batch of embryos not complying with the requirements of this Directive.
1.2. Embryos shall be collected in a place, which is separated from other parts of the premises or holding where the embryo is collected and which shall be in good repair and constructed with materials which permit its effective and easy cleansing and disinfection.
1.3. Embryos shall be processed (examined, washed, treated and placed in identified and sterile straws, ampoules or other packages) in either a permanently sited laboratory or a mobile laboratory, which, as regards susceptible species, is situated in an area in which there has been no outbreak of foot-and-mouth disease for the past 30 days within a 10 kilometre radius.
1.4. All equipment used to collect, handle, wash, freeze and store embryos shall either be sterilised or properly cleansed and disinfected prior to use according to the IETS Manual , or be single-use equipment.
1.5. Any biological product of animal origin used in the media and solutions for collection, processing, washing or storage of embryos shall be free of pathogenic micro-organisms. Media and solutions used in the collection, freezing and storage of embryos shall be sterilised by approved methods according to the IETS Manual and handled in such a manner as to ensure that sterility is maintained. Antibiotics might be added, when appropriate, to collection, processing, washing and storage media according to the IETS Manual.
1.6. The cryogenic agents used for preservation or storage of embryos shall not be previously used for other products of animal origin.
1.7. Each embryo straw, ampoule or other package shall be clearly identified by labels according to the standardised system according to the IETS Manual.
1.8. The embryos shall be washed and have an intact zona pellucida , or the embryonic capsule in case of equine embryos, before and immediately after washing. In accordance with the IETS Manual, the standard washing procedure shall be modified to include additional washes with the enzyme trypsin where recommended for the inactivation or removal of certain pathogens.
1.9. Embryos from different donor animals shall not be washed together.
1.10. The zona pellucida of each embryo, or the embryonic capsule in case of equine embryos, shall be examined over its entire surface area at not less than 50 × magnification and certified to be intact and free of adherent material.
1.11. Embryos of a batch that has successfully undergone the examination set out in point 1.10 shall be placed in a sterile straw, ampoule or other package marked in accordance with point 1.7 which shall be sealed immediately.
1.12. Each embryo shall, where appropriate, be frozen as soon as possible and stored in a place which is under the control of the team veterinarian.
1.13. Each embryo collection team shall submit for official examination for bacterial and viral contamination routine samples of non-viable embryos or ova, flushing fluids or washing fluids resulting from its activities according to the IETS Manual.
1.14. Each embryo collection team shall keep a record of its activities in respect of embryo collection for a period of two years after the embryos have been the subject of trade or import, including:
(a) the breed, age and individual identification of the donor animals concerned;
(b) the place of collection, processing and storage of embryos collected by the team;
(c) the identification of the embryos together with details of the consignee of the shipment.

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Source: https://www.legislation.gov.uk/eudr/1992/65/annex/D/chapter/III/division/II/division/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1992-65/annex-D/chapter-III/division-II/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.