# Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease, Division 3: Antibiotic medium

Extent: E+W+S+N.I.

Different laboratories have used various formulations of antibiotic medium with success and laboratories referred to in Annex II will be able to offer advice for a particular country. High concentrations of antibiotics are required for faeces samples and a typical mixture is: 10 000 units/ml penicillin, 10 mg/ml streptomycin, 0,25 mg/ml gentamycin and 5 000 units/ml mycostatin in phosphate buffered saline (PBS) These levels can be reduced up to five-fold for tissues and tracheal swabs. For control of Chlamydia organisms, 50 mg/ml oxytetracycline may be added. It is imperative when making the medium that the pH is checked after the addition of the antibiotics and readjusted to pH 7,0-7,4.

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Source: https://www.legislation.gov.uk/eudr/1992/66/annex/III/chapter/1/division/3 (the official version)
Page: https://openlegislation.uk/eu-directive-1992-66/annex-III/chapter-1/division-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.