{
  "url": "https://openlegislation.uk/eu-directive-1992-66/annex-III/chapter-2/division-1",
  "json_url": "https://openlegislation.uk/eu-directive-1992-66/annex-III/chapter-2/division-1.json",
  "legislation": "eudr/1992/66",
  "legislation_title": "Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease",
  "legislation_url": "https://openlegislation.uk/eu-directive-1992-66",
  "path": "annex/III/chapter/2/division/1",
  "kind": "division",
  "number": null,
  "heading": "Virus isolation in embryonated fowls' eggs",
  "text": "The clarified supernatent fluid should be inoculated in 0,1-0,2 ml amounts into the allantoic cavity of each of a minimum of four embryonated, fowls' eggs which have been incubated for 8 to 10 days. Ideally, these eggs should be obtained from a specific pathogen-free flock, but when this is impracticable it is acceptable to use eggs obtained from a flock shown to be free of antibodies to Newcastle-disease virus. The inoculated eggs are held at 37 °C and candled daily. Eggs with dead or dying embryos as they arise, and all remaining eggs six days after inoculation should be chilled to 4 °C and the allantoic-amniotic fluids tested for haemagglutination activity. If no haemagglutination is detected the above procedure is repeated using undiluted allantoic/amniotic fluid as inoculum.\nWhen haemagglutination is detected the presence of bacteria should be excluded by culture. If bacteria are present the fluids may be passed through a 450 nm membrane filter, further antibiotics added and inoculated into embryonated eggs as above.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1992/66/annex/III/chapter/2/division/1",
    "retrieved_at": "2026-11-01T15:23:21.646631+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}