# Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease, Division 5

Extent: E+W+S+N.I.

After 72 hours, incubation at 37 °C the plaques should be of sufficient size. They are best seen by removing the agar overlay and staining the cell monolayer with crystal violet (0,5 % w/v) in 25 % v/v ethanol.

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Source: https://www.legislation.gov.uk/eudr/1992/66/annex/III/chapter/8/division/5 (the official version)
Page: https://openlegislation.uk/eu-directive-1992-66/annex-III/chapter-8/division-5
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.