# Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot, Division 10.4

Extent: E+W+S+N.I.

Isolate from symptomatic plants by removing a section of stem 2 cm above the inoculation point. Comminute and suspend in a small volume of sterile distilled water or 50 mM phosphate buffer (Appendix 3). Isolate from the suspension by dilution spreading or streaking onto MTNA and YPGA (Appendix 5), incubate for three to five days at 21 to 23 °C and observe the formation of colonies typical of C. m. subsp. sepedonicus .

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Source: https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/10/division/10.4 (the official version)
Page: https://openlegislation.uk/eu-directive-1993-85/annex-I/division-10/division-10.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.