# Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot, Division 3.1.4

Extent: E+W+S+N.I.

Decant the supernatant. If excessively cloudy, clarify either by slow speed centrifugation (at not more than 180 g for 10 minutes at a temperature between 4 to 10 °C) or by vacuum filtration (40 to 100 µm), washing the filter with additional (10 ml) extraction buffer (Appendix 3).

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Source: https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/3/division/3.1/division/3.1.4 (the official version)
Page: https://openlegislation.uk/eu-directive-1993-85/annex-I/division-3/division-3.1/division-3.1.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.