# Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot, Division 3.1.6

Extent: E+W+S+N.I.

Resuspend the pellet in 1,5 ml pellet buffer (Appendix 3). Use 500 µl to test for C. m. subsp. sepedonicus , 500 µl for Ralstonia solanacearum and 500 µl for reference purposes. Add sterile glycerol to final concentration of 10 to 25 % (v/v) to the 500 µl of the reference aliquot and to the remaining test aliquot, vortex and store at -16 to -24 °C (weeks) or at 68 to -86 °C (months). Preserve the test aliquots at 4 to 10 °C during testing.
Repeated freezing and thawing is not advisable.
If transport of the extract is required, ensure delivery in a cool box within 24 to 48 hours.

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Source: https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/3/division/3.1/division/3.1.6 (the official version)
Page: https://openlegislation.uk/eu-directive-1993-85/annex-I/division-3/division-3.1/division-3.1.6
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.