# Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot, Division 3.2.2

Extent: E+W+S+N.I.

Process the stem segments by one of the following procedures: either,
(a) cover the segments with sufficient volume (approximately 40 ml) of extraction buffer (Appendix 3) and agitate on a rotary shaker (50 to 100 rpm) for four hours below 24 °C or for 16 to 24 hours refrigerated,
or
(b) process immediately. By crushing the segments in a strong maceration bag (e.g. Stomacher or Bioreba) with an appropriate volume of extraction buffer (Appendix 3) using a rubber mallet or appropriate grinding apparatus (e.g. Homex). If this is not possible, store the stem segments refrigerated for not longer than 72 hours or for not longer than 24 hours at room temperature.

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Source: https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/3/division/3.2/division/3.2.2 (the official version)
Page: https://openlegislation.uk/eu-directive-1993-85/annex-I/division-3/division-3.2/division-3.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.