{
  "url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-4/division-4.3/division-1",
  "json_url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-4/division-4.3/division-1.json",
  "legislation": "eudr/1993/85",
  "legislation_title": "Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot",
  "legislation_url": "https://openlegislation.uk/eu-directive-1993-85",
  "path": "annex/I/division/4/division/4.3/division/1",
  "kind": "division",
  "number": null,
  "heading": null,
  "text": "IF procedure:\n(i) According to test slide preparation in 4.1(i):\nPrepare a set of twofold dilutions of the antibody in IF buffer. The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).\n(ii) According to test slide preparation in 4.1(ii):\nPrepare the working dilution (WD) of the antibody in IF buffer. The working dilution affects the specificity.\nFigure 1.\nPreparation of the test slide according to 4.1(i) and 4.3(i)\nDilutions of resuspended pellet\n1/100\n1/1\n1/1\n1/1\n1/1\n\nDilution of resuspended pellet\n(T = titre)\nT/2\nT/4\nT/2\nT\n2T\n\nTwofold dilutions of antiserum/antibody\nSample 1\n1\n2\n3\n4\n5\nDuplicate of sample 1 or sample 2\n6\n7\n8\n9\n10\nFigure 2.\nPreparation of the test slide according to 4.1(ii) and 4.3(ii)\nWorking dilution of antiserum/antibody\n1/1\n1/10\n1/100\nempty\nempty\n\nDecimal dilution of resuspended pellet\nSample 1\n1\n2\n3\n4\n5\nDuplicate of sample 1 or sample 2\n6\n7\n8\n9\n10",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/4/division/4.3/division/1",
    "retrieved_at": "2026-11-01T15:23:17.858184+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}