# Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot, Division

Extent: E+W+S+N.I.

IF procedure:
(i) According to test slide preparation in 4.1(i):
Prepare a set of twofold dilutions of the antibody in IF buffer. The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).
(ii) According to test slide preparation in 4.1(ii):
Prepare the working dilution (WD) of the antibody in IF buffer. The working dilution affects the specificity.
Figure 1.
Preparation of the test slide according to 4.1(i) and 4.3(i)
Dilutions of resuspended pellet
1/100
1/1
1/1
1/1
1/1

Dilution of resuspended pellet
(T = titre)
T/2
T/4
T/2
T
2T

Twofold dilutions of antiserum/antibody
Sample 1
1
2
3
4
5
Duplicate of sample 1 or sample 2
6
7
8
9
10
Figure 2.
Preparation of the test slide according to 4.1(ii) and 4.3(ii)
Working dilution of antiserum/antibody
1/1
1/10
1/100
empty
empty

Decimal dilution of resuspended pellet
Sample 1
1
2
3
4
5
Duplicate of sample 1 or sample 2
6
7
8
9
10

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Source: https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/4/division/4.3/division/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1993-85/annex-I/division-4/division-4.3/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.