{
  "url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-5/division-5.1/division-5.1.5",
  "json_url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-5/division-5.1/division-5.1.5.json",
  "legislation": "eudr/1993/85",
  "legislation_title": "Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot",
  "legislation_url": "https://openlegislation.uk/eu-directive-1993-85",
  "path": "annex/I/division/5/division/5.1/division/5.1.5",
  "kind": "division",
  "number": "5.1.5",
  "heading": null,
  "text": "Spot 16 µl of the fixed suspensions onto a clean multitest slide as shown in Fig. 3. Apply two different samples per slide, undiluted and use 10 µl to make a 1:100 dilution (in 0,01M PB). The remaining sample solution (49 µl) can be stored at -20 °C after addition of 1 volume of 96 % ethanol. In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01M PB (mix by vortexing).\nFigure 3.\nLayout for FISH slide\nSample 1\nBlank\nBlank\nBlank\nSample 2\nwindow 1\nwindow 2\nwindow 3\nwindow 4\nwindow 5\nSample 1\nBlank\nBlank\nBlank\nSample 2\nwindow 6\nwindow 7\nwindow 8\nwindow 9\nwindow 10\nCoverslip 1\nCoverslip 2",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/5/division/5.1/division/5.1.5",
    "retrieved_at": "2026-11-01T15:23:17.858184+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}