{
  "url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-8/division-8.1/division-8.1.2",
  "json_url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-8/division-8.1/division-8.1.2.json",
  "legislation": "eudr/1993/85",
  "legislation_title": "Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot",
  "legislation_url": "https://openlegislation.uk/eu-directive-1993-85",
  "path": "annex/I/division/8/division/8.1/division/8.1.2",
  "kind": "division",
  "number": "8.1.2",
  "heading": null,
  "text": "Isolation from undiluted potato pellet usually fails due to the fastidious growth habit of Cms and competition by saprophytes. Since the bacterium is usually present in high populations in infected tissues, the saprophytes can usually be diluted out, whilst the pathogen remains. It is therefore recommended to spread 100 µl from each of the samples, 1/100 up to 1/ 10 000 dilutions onto MTNA medium or NCP-88 medium (Appendix 5) (if using 90 mm diameter petri dishes- adjust volume for alternative dish sizes), using spreaders (hockey sticks) and the spread plate technique.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/8/division/8.1/division/8.1.2",
    "retrieved_at": "2026-11-01T15:23:17.858184+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}