{
  "url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-9/division-9.1",
  "json_url": "https://openlegislation.uk/eu-directive-1993-85/annex-I/division-9/division-9.1.json",
  "legislation": "eudr/1993/85",
  "legislation_title": "Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot",
  "legislation_url": "https://openlegislation.uk/eu-directive-1993-85",
  "path": "annex/I/division/9/division/9.1",
  "kind": "division",
  "number": "9.1",
  "heading": "Nutritional and enzymatic identification tests",
  "text": "Determine the following phenotypic properties which are universally present or absent in C. m. subsp. sepedonicus , according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001), Anonymous (1987).\nAll media should be incubated at 21 °C and examined after six days. If no growth has occurred, incubate for up to 20 days.\nAll tests must include a known C. m. subsp. sepedonicus control. Nutritional and physiological tests must be made using inocula from nutrient agar subcultures. Morphological comparisons must be made from nutrient dextrose agar cultures.\nTests\nExpected result\nOxidation/Fermentation (O/F) test\nInert or weakly oxidative\nOxidase activity\n–\nGrowth at 37 °C\n–\nUrease activity\n–\nAesculin hydrolysis\n+\nStarch hydrolysis\n– or weak\nTolerance of 7 % NaCl\n–\nIndole production\n–\nCatalase activity\n+\nH 2 S production\n–\nCitrate utilization\n–\nGelatin liquefaction\n–\nAcid glycerol\n–\nAcid from lactose\n– or weak\nAcid from rhamnose\n–\nAcid from salicin\n–\nGram stain (Appendix 9)\n+",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1993/85/annex/I/division/9/division/9.1",
    "retrieved_at": "2026-11-01T15:23:17.858184+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}