{
  "url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/appendix-5",
  "json_url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/appendix-5.json",
  "legislation": "eudr/1998/57",
  "legislation_title": "Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.",
  "legislation_url": "https://openlegislation.uk/eu-directive-1998-57",
  "path": "annex/II/appendix/5",
  "kind": "appendix",
  "number": "5",
  "heading": "Determination of contamination level in IF and FISH tests",
  "text": "1.. Count the mean number of typical fluorescent cells per field of view (c).\n2.. Calculate the number of typical fluorescent cells per microscope slide window (C).\nC = c × S/s\nwhere\nS\n=\nsurface area of window of multispot slide\nand\ns\n=\nsurface area of objective field\ns = πi 2 /4G 2 K 2\nwhere\ni\n=\nfield coefficient (varies from 8 to 24 depending upon ocular type)\nK\n=\ntube coefficient (1 or 1,25)\nG\n=\nmagnification of objective (100 ×, 40 × etc.).\n3.. Calculate the number of typical fluorescent cells per ml of re-suspended pellet (N).\nN = C × 1 000 /y × F\nwhere\ny\n=\nvolume of re-suspended pellet on each window\nand\nF\n=\ndilution factor of re-suspended pellet.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1998/57/annex/II/appendix/5",
    "retrieved_at": "2026-11-01T15:23:06.931886+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}