{
  "url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/division-2",
  "json_url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/division-2.json",
  "legislation": "eudr/1998/57",
  "legislation_title": "Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.",
  "legislation_url": "https://openlegislation.uk/eu-directive-1998-57",
  "path": "annex/II/division/2",
  "kind": "division",
  "number": null,
  "heading": "CONTENTS",
  "text": "General principles\nSECTION I:\nApplication of the test scheme\n1.\nDetection scheme for the diagnosis of brown rot and bacterial wilt ( R. solanacearum ) in potato tubers and potato, tomato or other host plants with symptoms of brown rot or bacterial wilt\n2.\nScheme for detection and identification of R. solanacearum in samples of asymptomatic potato tubers\n3.\nScheme for detection and identification of R. solanacearum in samples of asymptomatic, potato, tomato or other host plants\nSECTION II:\nDetailed methods for detection of R. solanacearum in potato tubers and potato, tomato or other host plants with symptoms of brown rot or bacterial wilt\n1.\nSymptoms\n2.\nRapid screening tests\n3.\nIsolation procedure\n4.\nIdentification tests for R. solanacearum\nSECTION III:\n1.\nDetailed methods for detection and identification of R. solanacearum in samples of asymptomatic potato tubers\n1.1.\nSample preparation\n1.2.\nTesting\n2.\nDetailed methods for detection and identification of R. solanacearum in samples of asymptomatic potato, tomato or other plants\n2.1.\nSample preparation\n2.2.\nTesting\nSECTION IV:\n1.\nScheme for detection and identification of R. solanacearum in water\n2.\nMethods for detection and identification of R. solanacearum in water\n2.1.\nSample preparation\n2.2.\nTesting\nSECTION V:\n1.\nScheme for detection and identification of R. solanacearum in soil\n2.\nMethods for detection and identification of R. solanacearum in soil\n2.1.\nSamples preparation\n2.2.\nTesting\nSECTION VI:\nOptimised protocols for detection and identification of R. solanacearum\nA\nDiagnostic and detection tests\n1.\nStem streaming test\n2.\nDetection of poly-ß-hydroxybutyrate granules\n3.\nSerological agglutination tests\n4.\nSelective isolation\n4.1.\nSelective plating\n4.2.\nEnrichment procedure\n5.\nImmunofluorescence test (IF test)\n6.\nPolymerase chain reaction test (PCR test)\n6.1.\nDNA purification methods\n(a)\nMethod according to Pastrik (2000)\n(b)\nOther methods\n6.2.\nPCR\n6.3.\nAnalysis of the PCR product\n7.\nFluorescent in-situ hybridization test (FISH test)\n8.\nEnzyme Linked ImmunoSorbent Assay (ELISA) tests\n(a)\nIndirect ELISA\n(b)\nDASI (Double-Antibody Sandwich Indirect) ELISA\n9.\nBioassay test\nB\nIdentification tests\n1.\nNutritional and enzymatic identification tests\n2.\nIF test\n3.\nELISA test\n4.\nPCR test\n5.\nFISH test\n6.\nFatty acid profiling (FAP)\n7.\nStrain characterisation methods\n7.1.\nBiovar determination\n7.2.\nGenomic fingerprinting\n7.3.\nPCR methods\nC\nConfirmation test\nAppendix 1\nLaboratories involved in optimisation and validation of protocols\nAppendix 2\nMedia for isolation and culture of R. solanacearum\nAppendix 3\n(A)\nCommercially available standardised control material\n(B)\nPreparation of controls\nAppendix 4\nBuffers for test procedures\nAppendix 5\nDetermination of contamination level in IF and FISH tests\nAppendix 6\nValidated PCR protocols and reagents\nAppendix 7\nValidated reagents for FISH test\nAppendix 8\nCulture conditions for tomato and eggplant\nReferences",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1998/57/annex/II/division/2",
    "retrieved_at": "2026-11-01T15:23:06.931886+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}