# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 1.1.3

Extent: E+W+S+N.I.

Decant the supernatant. If excessively cloudy, clarify either by slow speed centrifugation (at not more than 180 g for 10 minutes at a temperature between 4 to 10 °C) or by vacuum filtration (40 to 100 µm), washing the filter with additional (approximately 10 ml) extraction buffer.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/III/division/1/division/1.1/division/1.1.3 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-III/division-1/division-1.1/division-1.1.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.