# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 1.1.5

Extent: E+W+S+N.I.

Resuspend the pellet in 1.5 ml pellet buffer (Appendix 4). Use 500 µl to test for R. solanacearum , 500 µl for Clavibacter michiganensis subsp. sepedonicus and 500 µl for reference purposes. Add sterile glycerol to final concentration of 10 to 25 % (v/v) to the 500 µl of the reference aliquot and to the remaining test aliquot, vortex and store at -16 to -24 °C (weeks) or at -68 to -86 °C (months). Preserve the test aliquots at 4 to 10 °C during testing.
Repeated freezing and thawing is not advisable.
If transport of the extract is required, ensure delivery in a cool box within 24 to 48 hours.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/III/division/1/division/1.1/division/1.1.5 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-III/division-1/division-1.1/division-1.1.5
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.