# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 2.1.2

Extent: E+W+S+N.I.

Disperse sub-samples of 10 to 25 g of soil or sludge by rotary shaking (250 rpm) in 60 to 150 ml extraction buffer (Appendix 4) for up to two hours. If required, addition of 0,02 % sterile Tween-20 and 10 to 20 g sterile gravel may assist dispersion.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/V/division/2/division/2.1/division/2.1.2 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-V/division-2/division-2.1/division-2.1.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.