# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division

Extent: E+W+S+N.I.

1.. Prepare a smear of bacterial ooze from infected tissue or from a 48-hour culture on YPGA or SPA medium (Appendix 2) on a microscope slide.
2.. Prepare positive control smears of a biovar 2 strain of R. solanacearum and, if considered useful, a negative control smear of a known PHB negative sp.
3.. Allow to air dry and pass the lower surface of each slide rapidly above a flame to fix the smears.
4.. Stain preparation with either Nile Blue or Sudan Black and observe microscopically as described below:

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/2/division/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-2/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.