{
  "url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-4/division-4.1/division-1/annotation/1",
  "json_url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-4/division-4.1/division-1/annotation/1.json",
  "legislation": "eudr/1998/57",
  "legislation_title": "Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.",
  "legislation_url": "https://openlegislation.uk/eu-directive-1998-57",
  "path": "annex/II/section/VI/division/A/division/4/division/4.1/division/1/annotation/1",
  "kind": "division",
  "number": null,
  "heading": null,
  "text": "Note: Before using this method for the first time, perform preliminary tests to ensure reproducible detection of 10 3 to 10 4 colony-forming units of R. solanacearum per ml added to extracts from samples which previously tested negative.\nUse an appropriately validated selective medium such as SMSA (as modified by Elphinstone et al. , 1996; see Appendix 2).\nCare is required to differentiate R. solanacearum from other bacteria able to develop colonies on the medium. Furthermore, colonies of R. solanacearum may show atypical morphology if plates are overcrowded or antagonistic bacteria are also present. Where effects of competition or antagonism are suspected, the sample should be re-tested using a different test.\nHighest sensitivity of detection by this method can be expected when using freshly prepared sample extracts. However, the method is also applicable for use with extracts which have been stored under glycerol at -68 to -86 °C.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/4/division/4.1/division/1/annotation/1",
    "retrieved_at": "2026-11-01T15:23:06.931886+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}