# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division

Extent: E+W+S+N.I.

Note: Before using this method for the first time, perform preliminary tests to ensure reproducible detection of 10 3 to 10 4 colony-forming units of R. solanacearum per ml added to extracts from samples which previously tested negative.
Use an appropriately validated selective medium such as SMSA (as modified by Elphinstone et al. , 1996; see Appendix 2).
Care is required to differentiate R. solanacearum from other bacteria able to develop colonies on the medium. Furthermore, colonies of R. solanacearum may show atypical morphology if plates are overcrowded or antagonistic bacteria are also present. Where effects of competition or antagonism are suspected, the sample should be re-tested using a different test.
Highest sensitivity of detection by this method can be expected when using freshly prepared sample extracts. However, the method is also applicable for use with extracts which have been stored under glycerol at -68 to -86 °C.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/4/division/4.1/division/1/annotation/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-4/division-4.1/division-1/annotation/1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.