# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division

Extent: E+W+S+N.I.

Use a validated enrichment medium such as modified Wilbrink broth (see Appendix 2).
This procedure can be used to selectively increase R. solanacearum populations in sample extracts and increase sensitivity of detection. The procedure also effectively dilutes inhibitors of the PCR reaction (1:100). It should be noted, however, that enrichment of R. solanacearum can fail due to competition or antagonism by saprophytic organisms which are often simultaneously enriched. For this reason, isolation of R.solanacearum from enriched broth cultures may be difficult. In addition, since populations of serologically related saprophytes can be increased, the use of specific monoclonal antibodies rather than polyclonal antibodies is recommended where the ELISA test is to be used.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/4/division/4.2/division/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-4/division-4.2/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.