{
  "url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-5/division-5.3/division-1",
  "json_url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-5/division-5.3/division-1.json",
  "legislation": "eudr/1998/57",
  "legislation_title": "Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.",
  "legislation_url": "https://openlegislation.uk/eu-directive-1998-57",
  "path": "annex/II/section/VI/division/A/division/5/division/5.3/division/1",
  "kind": "division",
  "number": null,
  "heading": null,
  "text": "(i) According to test slide preparation in 5.1(i):\nPrepare a set of twofold dilutions The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).\n(ii) According to test slide preparation in 5.1(ii):\nPrepare the working dilution (WD) of the antibody in IF buffer. The working dilution affects the specificity.\nFigure 1.\nPreparation of the test slide according to 5.1(i) and 5.3(i)\nDilutions of resuspended pellet\n1/100\n1/1\n1/1\n1/1\n1/1\n\nDilution of resuspended pellet\n(T = titre)\nT/2\nT/4\nT/2\nT\n2T\n\nTwofold dilutions of antiserum/antibody\nSample 1\n1\n2\n3\n4\n5\nDuplicate of sample1 or sample 2\n6\n7\n8\n9\n10\nFigure 2.\nPreparation of the test slide according to 5.1(ii) and 5.3(ii).\nWorking dilution of antiserum/antibody\n1/1\n1/10\n1/100\nempty\nempty\n\nDecimal dilution of resuspended pellet\nSample 1\n1\n2\n3\n4\n5\nDuplicate of sample 1 or sample 2\n6\n7\n8\n9\n10",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/5/division/5.3/division/1",
    "retrieved_at": "2026-11-01T15:23:06.931886+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}