# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 6.3.4

Extent: E+W+S+N.I.

For all new findings/cases verify authenticity of the PCR amplicon by performing restriction enzyme analysis on a sample of the remaining amplified DNA by incubating at the optimum temperature and time with an appropriate enzyme and buffer (see Appendix 6). Resolve the digested fragments by agarose gel electrophoresis as before and observe characteristic restriction fragment pattern under UV transillumination after ethidium bromide staining and compare with the undigested and digested positive control.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/6/division/6.3/division/6.3.4 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-6/division-6.3/division-6.3.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.