{
  "url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-7/division-7.1/division-7.1.5",
  "json_url": "https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-7/division-7.1/division-7.1.5.json",
  "legislation": "eudr/1998/57",
  "legislation_title": "Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.",
  "legislation_url": "https://openlegislation.uk/eu-directive-1998-57",
  "path": "annex/II/section/VI/division/A/division/7/division/7.1/division/7.1.5",
  "kind": "division",
  "number": "7.1.5",
  "heading": null,
  "text": "Spot 16 µl of the fixed suspensions onto a clean multitest slide as shown in Fig. 7.1. Applying two different samples per slide, undiluted and use 10 µl to make a 1:100 dilution (in 0,01 M PB). The remaining sample solution (49 µl) can be stored at -20 °C after addition of one volume of 96 % ethanol. In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01 PB (mix by vortexing).\nFig. 7.1\nLayout for FISH slide\nSample 1\nBlank\nBlank\nBlank\nSample 2\nwindow 1\nwindow 2\nwindow 3\nwindow 4\nwindow 5\nSample 1\nBlank\nBlank\nBlank\nSample 2\nwindow 6\nwindow 7\nwindow 8\nwindow 9\nwindow 10\nCoverslip 1\nCoverslip 2",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/7/division/7.1/division/7.1.5",
    "retrieved_at": "2026-11-01T15:23:06.931886+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}