# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 7.1.5

Extent: E+W+S+N.I.

Spot 16 µl of the fixed suspensions onto a clean multitest slide as shown in Fig. 7.1. Applying two different samples per slide, undiluted and use 10 µl to make a 1:100 dilution (in 0,01 M PB). The remaining sample solution (49 µl) can be stored at -20 °C after addition of one volume of 96 % ethanol. In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01 PB (mix by vortexing).
Fig. 7.1
Layout for FISH slide
Sample 1
Blank
Blank
Blank
Sample 2
window 1
window 2
window 3
window 4
window 5
Sample 1
Blank
Blank
Blank
Sample 2
window 6
window 7
window 8
window 9
window 10
Coverslip 1
Coverslip 2

---
Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/7/division/7.1/division/7.1.5 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-7/division-7.1/division-7.1.5
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.