# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 9.2.2: Slit inoculation

Extent: E+W+S+N.I.

Holding the plant between two fingers, pipette a drop (approximately 5 - 10 µl) of the suspended pellet on the stem between the cotyledons and the first leaf.
Using a sterile scalpel, make a diagonal slit, about 1.0 cm long and approximately 2/3 of the stem thickness deep, starting the cut from the pellet drop.
Seal the cut with sterile vaseline from a syringe.

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/A/division/9/division/9.2/division/9.2.2 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-A/division-9/division-9.2/division-9.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.