# Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al., Division 1: Nutritional and enzymatic identification tests

Extent: E+W+S+N.I.

Determine the following phenotypic properties, which are universally present or absent in R. solanacearum , according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001).
Test
Expected result
Fluorescent pigment production
–
Poly-ß-hydroxybutyrate inclusions
+
Oxidation/fermentation (O/F) test
O+/F–
Catalase activity
+
Kovac’s oxidase test
+
Reduction of nitrate
+
Utilisation of citrate
+
Growth at 40 °C
–
Growth in 1 % NaCl
+
Growth in 2 % NaCl
–
Arginine dihydrolase activity
–
Gelatine liquefaction
–
Starch hydrolysis
–
Aesculin hydrolysis
–
Levan production
–

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Source: https://www.legislation.gov.uk/eudr/1998/57/annex/II/section/VI/division/B/division/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1998-57/annex-II/section-VI/division-B/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.