# Commission Directive 2004/73/EC of 29 April 2004 adapting to technical progress for the twenty-ninth time Council Directive 67/548/EEC on the approximation of the laws, regulations and administrative provisions relating to the classification, packaging and labelling of dangerous substances (Text with EEA relevance), Division 1.3.3.2: Preparation of cell suspensions

Extent: E+W+S+N.I.

A single cell suspension of lymph node cells (LNC) either from pooled treatment groups or bilaterally from individual animals is prepared by gentle mechanical disaggregation through 200 µm-mesh stainless steel gauze. Lymph node cells are washed twice with an excess of PBS and precipitated with 5% trichloroacetic acid (TCA) at 4 oC for 18h (2). Pellets are either re-suspended in 1 ml TCA and transferred to scintillation vials containing 10 ml of scintillation fluid for 3H-counting, or transferred directly to gamma counting tubes for 125I-counting.

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Source: https://www.legislation.gov.uk/eudr/2004/73/annex/2H/division/B.42/division/1/division/1.3/division/1.3.3/division/1.3.3.2 (the official version)
Page: https://openlegislation.uk/eu-directive-2004-73/annex-2H/division-B.42/division-1/division-1.3/division-1.3.3/division-1.3.3.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.