# Commission Directive 2004/73/EC of 29 April 2004 adapting to technical progress for the twenty-ninth time Council Directive 67/548/EEC on the approximation of the laws, regulations and administrative provisions relating to the classification, packaging and labelling of dangerous substances (Text with EEA relevance), Division 1.8.2: Selection and number of aquatic sediments

Extent: E+W+S+N.I.

The sampling sites should be selected in accordance with the purpose of the test in any given situation. In selecting sampling sites, the history of possible agricultural, industrial or domestic inputs to the catchment and the waters upstream must be considered. Sediments should not be used if they have been contaminated with the test substance or its structural analogues within the previous 4 years.
Measurement of parameters for characterisation of water-sediment samples (7)(22)(23)
Recent research results have shown that measurements of water oxygen concentrations and of redox potentials have neither a mechanistic nor a predictive value as far as growth and development of microbial populations in surface waters are concerned (24)(25). Determination of the biochemical oxygen demand (BOD, at field sampling, start and end of test) and of concentrations of micro/macro nutrients Ca, Mg and Mn (at start and end of test) in water and the measurement of total N and total P in sediments (at field sampling and end of test) may be better tools to interpret and evaluate aerobic biotransformation rates and routes.
Microbial respiration rate method (26), fumigation method (27) or plate count measurements (e.g. bacteria, actinomycetes, fungi and total colonies) for aerobic studies; methanogenesis rate for anaerobic studies.
Parameter
Stage of test procedure
field sampling
post-handling
start of acclimation
start of test
during test
end of test
Water
Origin/source
X
Temperature
X
PH
X
X
X
X
X
TOC
X
X
X
O2 concentration
X
X
X
X
X
Redox Potential
X
X
X
X
Sediment
Origin/source
X
Depth of layer
X
PH
X
X
X
X
X
Particle size distribution
X
TOC
X
X
X
X
Microbial biomass
X
X
X
Redox potential
Observation (colour/smell)
X
X
X
X

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Source: https://www.legislation.gov.uk/eudr/2004/73/annex/2I/division/C.24/division/1/division/1.8/division/1.8.2 (the official version)
Page: https://openlegislation.uk/eu-directive-2004-73/annex-2I/division-C.24/division-1/division-1.8/division-1.8.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.