{
  "url": "https://openlegislation.uk/eu-directive-2006-56/annex-I/appendix-4",
  "json_url": "https://openlegislation.uk/eu-directive-2006-56/annex-I/appendix-4.json",
  "legislation": "eudr/2006/56",
  "legislation_title": "Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot",
  "legislation_url": "https://openlegislation.uk/eu-directive-2006-56",
  "path": "annex/I/appendix/4",
  "kind": "appendix",
  "number": "4",
  "heading": "Determination of contamination level in IF and FISH tests",
  "text": "1.. Count the mean number of typical fluorescent cells per field of view (c)\n2.. Calculate the number of typical fluorescent cells per microscope slide window (C)\nC = c × S/s\nwhere\nS\n=\nsurface area of window of multispot slide, and\ns\n=\nsurface area of objective field.\ns = πi2/4G2K2\nwhere\ni\n=\nfield coefficient (varies from 8 to 24 depending upon ocular type)\nK\n=\ntube coefficient (1 or 1,25)\nG\n=\nmagnification of objective (100x, 40x etc.).\n3.. Calculate the number of typical fluorescent cells per ml of resuspended pellet (N)\nN = C × 1 000/y × F\nwhere\ny\n=\nvolume of resuspended pellet on each window, and\nF\n=\ndilution factor of resuspended pellet.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/2006/56/annex/I/appendix/4",
    "retrieved_at": "2026-11-01T15:22:35.204179+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}