# Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot, Division 5.1.3

Extent: E+W+S+N.I.

Remove the supernatant and dissolve the pellet in 500 µl of fixative prepared < 24 hours previously. Vortex and incubate overnight at 4 °C.
An alternative fixative is 96 % ethanol. To use this dissolve the pellet from step 5.1.2 in 50 µl 0,01M PB and 50 µl 96 % ethanol. Vortex mix and incubate at 4 °C for 30 to 60 minutes.

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Source: https://www.legislation.gov.uk/eudr/2006/56/annex/I/division/5/division/5.1/division/5.1.3 (the official version)
Page: https://openlegislation.uk/eu-directive-2006-56/annex-I/division-5/division-5.1/division-5.1.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.