# Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot, Division 5.1.5

Extent: E+W+S+N.I.

Spot 16 µl of the fixed suspensions onto a clean multitest slide as shown in Fig. 3. Apply two different samples per slide, undiluted and use 10 µl to make a 1:100 dilution (in 0,01M PB). The remaining sample solution (49 µl) can be stored at -20 °C after addition of 1 volume of 96 % ethanol. In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01M PB (mix by vortexing).
Figure 3.
Layout for FISH slide
Sample 1
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Sample 2
window 1
window 2
window 3
window 4
window 5
Sample 1
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Sample 2
window 6
window 7
window 8
window 9
window 10
Coverslip 1
Coverslip 2

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Source: https://www.legislation.gov.uk/eudr/2006/56/annex/I/division/5/division/5.1/division/5.1.5 (the official version)
Page: https://openlegislation.uk/eu-directive-2006-56/annex-I/division-5/division-5.1/division-5.1.5
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.