# Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot, Division 7.10

Extent: E+W+S+N.I.

Under certain circumstances, in particular where growing conditions are not optimal, it may be possible for C. m. subsp. sepedonicus to exist as a latent infection within eggplants even after incubation periods up to 4 weeks. If no symptoms are observed after 4 weeks perform IF/PCR on a composite sample of 1 cm stem sections of each test plant taken above the inoculation site. If the test is positive reisolation on suitable (selective) media should be performed following the procedure under section 8. Identify purified cultures of presumptive C. m. subsp. sepedonicus and confirm pathogenicity (section 9 and 10).
Interpretation of the bioassay test result.
Valid Bioassay test results are obtained when plants of the positive control show typical symptoms, the bacteria can be reisolated from these plants and no symptoms are found on the negative controls.
The bioassay test is negative if test plants are not infected by C. m. subsp. sepedonicus, and provided that C. m. subsp. sepedonicus is detected in positive controls.
The bioassay test is positive if the test plants are infected by C. m. subsp. sepedonicus.

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Source: https://www.legislation.gov.uk/eudr/2006/56/annex/I/division/7/division/7.10 (the official version)
Page: https://openlegislation.uk/eu-directive-2006-56/annex-I/division-7/division-7.10
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.