{
  "url": "https://openlegislation.uk/eu-directive-2006-56/annex-I/division-9/division-9.1",
  "json_url": "https://openlegislation.uk/eu-directive-2006-56/annex-I/division-9/division-9.1.json",
  "legislation": "eudr/2006/56",
  "legislation_title": "Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot",
  "legislation_url": "https://openlegislation.uk/eu-directive-2006-56",
  "path": "annex/I/division/9/division/9.1",
  "kind": "division",
  "number": "9.1",
  "heading": "Nutritional and enzymatic identification tests",
  "text": "Determine the following phenotypic properties which are universally present or absent in C. m. subsp. sepedonicus, according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001), Anonymous (1987).\nAll media should be incubated at 21 °C and examined after six days. If no growth has occurred, incubate for up to 20 days.\nAll tests must include a known C. m. subsp.sepedonicus control. Nutritional and physiological tests must be made using inocula from nutrient agar subcultures. Morphological comparisons must be made from nutrient dextrose agar cultures.\nTests\nExpected result\nOxidation/Fermentation (O/F) test\nInert or weakly oxidative\nOxidase activity\n–\nGrowth at 37 °C\n–\nUrease activity\n–\nAesculin hydrolysis\n+\nStarch hydrolysis\n– or weak\nTolerance of 7 % NaCl\n–\nIndole production\n–\nCatalase activity\n+\nH2S production\n–\nCitrate utilization\n–\nGelatin liquefaction\n–\nAcid glycerol\n–\nAcid from lactose\n– or weak\nAcid from rhamnose\n–\nAcid from salicin\n–\nGram stain (Appendix 9)\n+",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/2006/56/annex/I/division/9/division/9.1",
    "retrieved_at": "2026-11-01T15:22:35.204179+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}